Influence of extremely low frequency magnetic fields on Ca2+ signaling

Bron: www.sciencedirect.com/science/article/pii/S0304394006012833

Neuroscience Letters
Volume 413, Issue 2, 14 February 2007, Pages 145–149

Influence of extremely low frequency magnetic fields on Ca2+ signaling and NMDA receptor functions in rat hippocampus

Pavan K. Manikondaa, P. Rajendrab, D. Devendranathb, B. Gunasekaranb, Channakeshavab, R.S.S. Aradhyab, R.B. Sashidhara, C. Subramanyama, ,
a Department of Biochemistry, Osmania University, Hyderabad 500007, India
b Ultra High Voltage Research Laboratory, Central Power Research Institute, Hyderabad 500039, India
Received 3 July 2006, Revised 20 November 2006, Accepted 21 November 2006, Available online 28 December 2006

Abstract
Extremely low frequency (ELF < 300 Hz) electromagnetic fields affect several neuronal activities including memory. Because ELF magnetic fields cause altered Ca2+ homeostasis in neural tissues, we examined their influence on Ca2+ signaling enzymes in hippocampus and related them with NMDA receptor functions. Hippocampal regions were obtained from brains of 21-day-old rats that were exposed for 90 days to 50 Hz magnetic fields at 50 and 100 μT intensities. In comparison to controls, ELF exposure caused increased intracellular Ca2+ levels concomitant with increased activities of Ca2+-dependent protein kinase C (PKC), cAMP-dependent protein kinase and calcineurin as well as decreased activity of Ca2+–calmodulin-dependent protein kinase in hippocampal regions. Simultaneous ligand-binding studies revealed decreased binding to N-methyl-d-aspartic acid (NMDA) receptors. The combined results suggest that perturbed neuronal functions caused by ELF exposure may involve altered Ca2+ signaling events contributing to aberrant NMDA receptor activities.

Keywords
Ca2+ signaling; Electromagnetic fields; Hippocampus; Memory; NMDA receptor



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