Rat testicular proteome following 30-days exposure to 900 MHz electromagnetic field radiation.

Bron: www.ncbi.nlm.nih.gov/pubmed/25146694 .
21 aug. 2014
Electrophoresis. 2014 Aug 21. doi: 10.1002/elps.201400273. (Epub ahead of print)

Analysis of rat testicular proteome following 30-days exposure to 900 MHz electromagnetic field radiation.

Sepehrimanesh M1, Kazemipour N, Saeb M, Nazifi S.
1Department of Biochemistry, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Abstract
The use of electromagnetic field (EMF) generating apparatuses such as cell phones is increasing, and has caused an interest in the investigations of its effects on human health. We analyzed proteome in preparations from the whole testis in adult male Sprague-Dawley rats exposed for 1, 2 or 4 h/d for 30 consecutive days to 900 MHz EMF radiation, simulating a range of possible human cell phone use. Subjects were sacrificed immediately after the end of the experiment and testes fractions were solubilized and separated via high resolution 2-dimensional electrophoresis, and gel patterns were scanned, digitized and processed. Thirteen of the proteins which found only in sham or in exposure groups were identified by MALDI-TOF/TOF-MS. Among them, heat shock proteins, superoxide dismutase, peroxiredoxin-1 and other proteins related to misfolding of proteins and/or stress were identified. These results demonstrate significant effects of radio-frequency modulated electromagnetic fields (RF-EMF) exposure on proteome, particularly in protein species in the rodent testis, and suggest that a 30 d exposure to EMF radiation induces non-thermal stress in testicular tissue. The functional implication of the identified proteins was discussed. This article is protected by copyright. All rights reserved.

KEYWORDS:
Heat shock proteins; Peroxiredoxin-1; Proteomic; RF-EMF; Superoxide dismutase



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