China: UMTS straling veroozaakt directe stressreactie in hippocampus bij ratten.
zaterdag, 21 april 2012 - Categorie: Onderzoeken
Brain Research Bulletin. 2012 Apr 9.
Exposure to 2.45GHz electromagnetic fields elicits an HSP-related stress response in rat hippocampus.
Yang XS, He GL, Hao YT, Xiao Y, Chen CH, Zhang GB, Yu ZP.
Key Laboratory of Medical Protection for Electromagnetic Radiation, Ministry of Education, Third Military Medical University, Chongqing 400038, PR China;
Institute of Tropical Medicine, Third Military Medical University, Chongqing 400038, PR China.
The issue of possible neurobiological effects of the electromagnetic field (EMF) exposure is highly controversial. To determine whether electromagnetic field exposure could act as an environmental stimulus capable of producing stress responses, we employed the hippocampus, a sensitive target of electromagnetic radiation, to assess the changes in its stress-related gene and protein expression after EMF exposure.
Adult male Sprague-Dawley rats with body restrained were exposed to a 2.45GHz EMF at a specific absorption rate (SAR) of 6W/kg or sham conditions. cDNA microarray was performed to examine the changes of gene expression involved in the biological effects of electromagnetic radiation. Of 2048 candidate genes, 23 upregulated and 18 downregulated genes were identified. Of these differential expression genes, two heat shock proteins (HSP), HSP27 and HSP70, are notable because expression levels of both proteins are increased in the rat hippocampus.
Result from immunocytochemistry revealed that EMF caused intensive staining for HSP27 and HSP70 in the hippocampus, especially in the pyramidal neurons of cornu ammonis 3 (CA3) and granular cells of dentate gyrus (DG). The gene and protein expression profiles of HSP27 and HSP70 were further confirmed by reverse transcription polymerase chain reaction (RT-PCR) and Western blot.
Our data provide direct evidence that exposure to electromagnetic fields elicits a stress response in the rat hippocampus.
Voor het originele abstract zie:
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