Extremely low frequency magnetic fields cause oxidative DNA damage in rats.

donderdag, 06 november 2008 - Categorie: Onderzoeken

Extremely low frequency magnetic fields cause oxidative DNA damage in rats.

Yokus B, Akdag MZ, Dasdag S, Cakir DU, Kizil M.

Department of Biochemistry, Faculty of Veterinary Medicine.

Purpose: To detect the genotoxic effects of extremely low frequency (ELF) -magnetic fields (MF) on oxidative DNA base modifications 8-hydroxyguanine (8-OH-Gua), 2,6-diamino-4-hydroxy-5-formamidopyrimidine (FapyGua) and 4,6-diamino-5-formamidopyrimidine (FapyAde) in rat leucocytes, measured following exposure to ELF-MF. Materials and methods: After exposure to ELF-MF (50 Hz, 100 and 500 muT, for 2 hours/day during 10 months), DNA was extracted, and measurement of DNA lesions was achieved by gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS). Results: Levels of FapyAde, FapyGua and 8OHdG in DNA were increased by both 100 muT and 500 muT ELF-MF as compared to a cage-control and a sham group; however, statistical significance was observed only in the group exposed to 100 muT. Conclusion: This is the first study to report that ELF-MF exposure generates oxidatively induced DNA base modifications which are mutagenic in mammalian cells, such as FapyGua, FapyAde and 8-OH-Gua, in vivo. This may explain previous studies showing DNA damage and genomic instability. These findings support the hypothesis that chronic exposure to 50-Hz MF may be potentially genotoxic. However, the intensity of ELF-MF has an important influence on the extent of DNA damage.

PMID: 18979312 PubMed - in process

Voor het originele abstract zie:
www.ncbi.nlm.nih.gov/pubmed/18979312?dopt=Abstract .

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