Celdood in het laboratorium
zaterdag, 12 januari 2008 - Categorie: Onderzoeken
Celdood in het laboratorium (in vitro), 900 MHz, 2 W/kg, 24 uur. Cellen van het zenuwstelsel.
Joubert, V., Bourthoumieu, S., Leveque, P. and Yardin, C. Apoptosis is Induced by Radiofrequency Fields through the Caspase-Independent Mitochondrial Pathway in Cortical Neurons. Radiat. Res. 169, 38–45 (2008).
Volume 169, Issue 1 (January 2008)
Article: pp. 38–45 | Full Text | PDF (1.01M)
Apoptosis is Induced by Radiofrequency Fields through the Caspase-Independent Mitochondrial Pathway in Cortical Neurons
Vanessa JoubertA, Sylvie BourthoumieuA, Philippe LevequeB, and Catherine YardinA
A. Department of Cell Biology, Histology and Cytogenetics, EA3842 Limoges University Hospital, Faculty of Medicine, 87025 Limoges Cedex, France, B. XLIM Department OSA, UMR CNRS 6172, Faculty of Sciences, 87060 Limoges Cedex, France
In the present study, we investigated whether continuous-wave (CW) radiofrequency (RF) fields induce neuron apoptosis in vitro. Rat primary neuronal cultures were exposed to a CW 900 MHz RF field with a specific absorption rate (SAR) of 2 W/kg for 24 h. During exposure, an increase of 2°C was measured in the medium; control experiments with neurons exposed to 39°C were then performed. Apoptosis was assessed by condensation of nuclei with 4′,6-diamino-2-phenylindole (DAPI) staining observed with an epifluorescence microscope and fragmentation of DNA with TdT-mediated dUTP nick-end labeling (TUNEL) analyzed by flow cytometry. A statistically significant difference in the rate of apoptosis was found in the RF-field-exposed neurons compared to the sham-, 37°C- and 39°C-exposed neurons either 0 or 24 h after exposure using both methods. To assess whether the observed apoptosis was caspase-dependent or -independent, assays measuring caspase 3 activity and apoptosis-inducing factor (AIF) labeling were performed. No increase in the caspase 3 activity was found, whereas the percentage of AIF-positive nuclei in RF-field-exposed neurons was increased by three- to sevenfold compared to other conditions. Our results show that, under the experimental conditions used, exposure of primary rat neurons to CW RF fields may induce a caspase-independent pathway to apoptosis that involves AIF.
Received: April 27, 2007; Accepted: August 29, 2007
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