High efficiency gene transfection by electroporation using a radio-frequency electric field.
vrijdag, 14 februari 2020 - Categorie: Onderzoeken
Biochim Biophys Acta. 1991 Apr 17;1092(2):153-60.
Chang DC 1, Gao PQ, Maxwell BL.
1. Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX 77030.
Een publicatie uit 1991, relevant omdat het dus toen al bekend was dat cel membranen permeabel worden door RF elektromagnetische straling, dus ook permeabel voor toxische stoffen.
In order to develop a safe and effective way to introduce exogenous genes into cells, we have experimented with a new method of electroporation which uses a radio-frequency (RF) electric field to permeabilize the cell membrane. This RF method has several advantages over the conventional electroporation method which uses a direct current (DC) field. We have shown that the RF electroporation method can be used to introduce marker genes into a wide variety of cell lines, including COS-M6, CV-1, CHO, 3T3 and hepatocytes, and is able to increase substantially the efficiency of gene transfection. (For example, the amount of DNA required for transfecting two million COS-M6 cells can be as low as 0.1 microgram). The transfection efficiency is shown to be affected by a number of factors, including cell type, field strength, pulse protocol and medium buffer. Because of its wide range of applications, high transfection efficiency and lack of harmful side-effect, the RF electroporation method would be particularly useful for introducing genes into human cells for gene therapy.
PMID: 1902117 DOI: 10.1016/0167-4889(91)90149-r
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