Semi-quantitative proteomics of mammalian cells upon short-term exposure to non-ionizing EMF
woensdag, 11 april 2018 - Categorie: Onderzoeken
PLoS One. 2017 Feb 24;12(2):e0170762. doi: 10.1371/journal.pone.0170762. eCollection 2017
Semi-quantitative proteomics of mammalian cells upon short-term exposure to non-ionizing electromagnetic fields.
Kuzniar A1,2, Laffeber C1, Eppink B1, Bezstarosti K3, Dekkers D3, Woelders H4, Zwamborn AP5, Demmers J3,6, Lebbink JH1,7, Kanaar R1,7.
1 Department of Molecular Genetics, Cancer Genomics Netherlands, Erasmus University Medical Center, Rotterdam, The Netherlands.
2 Netherlands eScience Center, Amsterdam, The Netherlands.
3 Proteomics Center, Erasmus University Medical Center, Rotterdam, The Netherlands.
4 Wageningen Livestock Research, Wageningen, The Netherlands.
5 TNO, The Hague, The Netherlands.
6 Netherlands Proteomics Center, Rotterdam, The Netherlands.
7 Department of Radiation Oncology, Erasmus University Medical Center, Rotterdam, The Netherlands.
The potential effects of non-ionizing electromagnetic fields (EMFs), such as those emitted by power-lines (in extremely low frequency range), mobile cellular systems and wireless networking devices (in radio frequency range) on human health have been intensively researched and debated. However, how exposure to these EMFs may lead to biological changes underlying possible health effects is still unclear. To reveal EMF-induced molecular changes, unbiased experiments (without a priori focusing on specific biological processes) with sensitive readouts are required. We present the first proteome-wide semi-quantitative mass spectrometry analysis of human fibroblasts, osteosarcomas and mouse embryonic stem cells exposed to three types of non-ionizing EMFs (ELF 50 Hz, UMTS 2.1 GHz and WiFi 5.8 GHz). We performed controlled in vitro EMF exposures of metabolically labeled mammalian cells followed by reliable statistical analyses of differential protein- and pathway-level regulations using an array of established bioinformatics methods. Our results indicate that less than 1% of the quantitated human or mouse proteome responds to the EMFs by small changes in protein abundance. Further network-based analysis of the differentially regulated proteins did not detect significantly perturbed cellular processes or pathways in human and mouse cells in response to ELF, UMTS or WiFi exposure. In conclusion, our extensive bioinformatics analyses of semi-quantitative mass spectrometry data do not support the notion that the short-time exposures to non-ionizing EMFs have a consistent biologically significant bearing on mammalian cells in culture.
Geen significant effect bij 'short-time exposure', maar hoe zit 't met 'long-time exposure' waar veel mensen tegenwoordig aan zijn blootgesteld?
Lees verder in de categorie Onderzoeken | Terug naar homepage | Lees de introductie