Effect RF straling op menselijke bindweefsel cellen
dinsdag, 04 juni 2013 - Categorie: Artikelen
Bron: www.ncbi.nlm.nih.gov/pubmed/23723005?dopt=Abstract .
31 mei 2013
Cell Biochem Biophys. 2013 May 31. (Epub ahead of print)
Investigation of the Effects of 2.1 GHz Microwave Radiation on Mitochondrial Membrane Potential (ΔΨ m), Apoptotic Activity and Cell Viability in Human Breast Fibroblast Cells.
Esmekaya MA, Seyhan N, Kayhan H, Tuysuz MZ, Kurşun AC, Yağcı M.
Department of Biophysics, Faculty of Medicine and Gazi Non-ionizing Radiation Protection (GNRP) Center, Gazi University, Beşevler, 06510, Ankara, Turkey, email@example.com.
In the present study we aimed to investigate the effects of 2.1 GHz Wideband Code Division Multiple Access (W-CDMA) modulated Microwave (MW) Radiation on cell survival and apoptotic activity of human breast fibroblast cells. The cell cultures were exposed to W-CDMA modulated MW at 2.1 GHz at a SAR level of 0.607 W/kg for 4 and 24 h. The cell viability was assessed by MTT 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. The percentage of apoptotic cells was analyzed by Annexin V-FITC and PI staining. 5,5',6,6'-Tetrachloro-1,1',3,3'- tetraethylbenzimidazolcarbocyanine iodide (JC-1) was used to measure Mitochondrial Membrane Potential (ΔΨ m). sFasL and Fas/APO-1 protein levels were determined by ELISA method. 2.1 GHz MW radiation was shown to be able to inhibit cell proliferation and induce apoptosis in human breast fibroblast cells. The cell viability of MW-exposed cells was decreased significantly. The percentages of Annexin V-FITC positive cells were higher in MW groups. ΔΨ m was decreased significantly due to MW radiation exposure. However, neither sFas nor FasL level was significantly changed in MW-exposed fibroblast cells. The results of this study showed that 2.1 GHz W-CDMA modulated MW radiation-induced apoptotic cell death via the mitochondrial pathway.
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